**** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION ****

MSDS Name: Indigo Carmine
Catalog Numbers:
    S71306, I166 25, I166-25, I16625
Synonyms:
    Carmine Blue (Biological Stain), C.I. 73015, C.I.75781, C.I. Acid
    Blue 74, C.I. Food Blue 1, C.I. Natural Blue 2, FD&C Blue 2, Ind
Company Identification:  Fisher Scientific
                        1 Reagent Lane
                        Fairlawn, NJ  07410
For information, call:  201-796-7100
Emergency Number:       201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887

         **** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
|      CAS#      |              Chemical Name           |    %     |  EINECS#  |
|----------------|--------------------------------------|----------|-----------|
|      860-22-0  |C.I. Acid Blue 74                     | ca. 100  | 212-728-8 |
+----------------+--------------------------------------+----------+-----------+
         Hazard Symbols: XN
         Risk Phrases: 22

                 **** SECTION 3 - HAZARDS IDENTIFICATION ****

                              EMERGENCY OVERVIEW
Appearance:  dark blue.
Caution! May be harmful if swallowed. May cause eye and skin
irritation. May cause respiratory and digestive tract irritation.
Target Organs: None.

Potential Health Effects
    Eye:
         This product contains a cationic dye. Similar dyes have caused
         permanent injury to the cornea and conjunctiva in documented
         exposure cases with human or rabbit eyes.
    Skin:
         May cause skin irritation.
    Ingestion:
         May cause gastrointestinal irritation with nausea, vomiting and
         diarrhea. May be harmful if swallowed.
    Inhalation:
         May cause respiratory tract irritation.
    Chronic:
         No information found.

                   **** SECTION 4 - FIRST AID MEASURES ****

    Eyes:
         Flush eyes with plenty of water for at least 15 minutes,
         occasionally lifting the upper and lower eyelids. Get medical aid.
         SPEEDY ACTION IS CRITICAL!
    Skin:
         Get medical aid if irritation develops or persists. Flush skin with
         plenty of soap and water.
    Ingestion:
         If victim is conscious and alert, give 2-4 cupfuls of milk or water.
         Never give anything by mouth to an unconscious person. Get medical
         aid.
    Inhalation:
         Remove from exposure to fresh air immediately. If not breathing,
         give artificial respiration. If breathing is difficult, give oxygen.
         Get medical aid.
    Notes to Physician:
         Treat symptomatically and
    Antidote:
         None reported.

                 **** SECTION 5 - FIRE FIGHTING MEASURES ****

    General Information:
         As in any fire, wear a self-contained breathing apparatus in
         pressure-demand, MSHA/NIOSH (approved or equivalent), and full
         protective gear.
    Extinguishing Media:
         In case of fire, use water, dry chemical, chemical foam, or
         alcohol-resistant foam.
    Autoignition Temperature:Not available.
    Flash Point:           Not available.
    Explosion Limits, lower:Not available.
    Explosion Limits, upper:Not available.
    NFPA Rating: (estimated) Health: 1; Flammability: 0; Reactivity: 0

               **** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

    General Information: Use proper personal protective equipment as indicated
                         in Section 8.
    Spills/Leaks:
         Clean up spills immediately, observing precautions in the Protective
         Equipment section. Sweep up or absorb material, then place into a
         suitable clean, dry, closed container for disposal. Avoid generating
         dusty conditions. Provide ventilation.

                  **** SECTION 7 - HANDLING and STORAGE ****

    Handling:
         Wash thoroughly after handling. Remove contaminated clothing and
         wash before reuse. Use with adequate ventilation. Avoid contact with
         eyes, skin, and clothing. Keep container tightly closed. Avoid
         ingestion and inhalation.
    Storage:
         Store in a cool, dry, well-ventilated area away from incompatible
         substances. Keep containers tightly closed.

         **** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

    Engineering Controls:
         Use adequate ventilation to keep airborne concentrations low.

                                Exposure Limits
+--------------------+-------------------+-------------------+-----------------+
|   Chemical Name    |        ACGIH      |       NIOSH       |OSHA - Final PELs|
|--------------------|-------------------|-------------------|-----------------|
| C.I. Acid Blue 74  |none listed        |none listed        |none listed      |
+--------------------+-------------------+-------------------+-----------------+

    OSHA Vacated PELs:
         C.I. Acid Blue 74:
            No OSHA Vacated PELs are listed for this chemical.

    Personal Protective Equipment

                Eyes:
                      Wear appropriate protective eyeglasses or chemical
                      safety goggles as described by OSHA's eye and face
                      protection regulations in 29 CFR 1910.133 or European
                      Standard EN166.
                Skin:
                      Wear appropriate protective gloves to prevent skin
                      exposure.
            Clothing:
                      Wear appropriate protective clothing to prevent skin
                      exposure.
         Respirators:
                      A respiratory protection program that meets OSHA's 29
                      CFR :1910.134 and ANSI Z88.2 requirements or European
                      Standard EN 149 must be followed whenever workplace
                      conditions warrant a respirator's use.

            **** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State:             Solid
Appearance:                 dark blue
Odor:                       none reported
pH:                         Not available.
Vapor Pressure:             Negligible.
Vapor Density:              Not available.
Evaporation Rate:           Negligible.
Viscosity:                  Not available.
Boiling Point:              Not available.
Freezing/Melting Point:     > 350 deg C
Decomposition Temperature:  Not available.
Solubility in water:        Slightly soluble in water.
Specific Gravity/Density:   Not available.
Molecular Formula:          C16H8N2O8S2Na2
Molecular Weight:           466.1642

                **** SECTION 10 - STABILITY AND REACTIVITY ****

    Chemical Stability:
         Stable under normal temperatures and pressures.
    Conditions to Avoid:
         Incompatible materials, dust generation.
    Incompatibilities with Other Materials:
         Strong oxidizing agents.
    Hazardous Decomposition Products:
         Nitrogen oxides, carbon monoxide, carbon dioxide, nitrogen.
    Hazardous Polymerization: Will not occur.

               **** SECTION 11 - TOXICOLOGICAL INFORMATION ****

    RTECS#:
         CAS# 860-22-0: DU3000000
    LD50/LC50:
         CAS# 860-22-0: Oral, mouse: LD50 = 2500 mg/kg; Oral, rat: LD50 = 2
         gm/kg.
    Carcinogenicity:
      C.I. Acid Blue 74 -
         Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
    Epidemiology:
         Tumorigenic data exists for indigo carmine.
    Teratogenicity:
         No information found.
    Reproductive Effects:
         No information found.
    Neurotoxicity:
         No information found.
    Mutagenicity:
         Mutagenicity studies have been performed with laboratory animals.
    Other Studies:
         No data available.

                 **** SECTION 12 - ECOLOGICAL INFORMATION ****

    Other
         No information available.

                **** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Chemical waste generators must determine whether a discarded chemical
is classified as a hazardous waste.
US EPA guidelines for the classification determination are listed in
40 CFR Parts 261.3. Additionally, waste generators must consult state
and local hazardous waste regulations to ensure complete and accurate
classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

                 **** SECTION 14 - TRANSPORT INFORMATION ****

    US DOT
         No information available
    Canadian TDG
         No information available.

                 **** SECTION 15 - REGULATORY INFORMATION ****

US FEDERAL
    TSCA
         CAS# 860-22-0 is listed on the TSCA inventory.
       Health & Safety Reporting List
         None of the chemicals are on the Health & Safety Reporting List.
       Chemical Test Rules
         None of the chemicals in this product are under a Chemical Test Rule.
       Section 12b
         None of the chemicals are listed under TSCA Section 12b.
       TSCA Significant New Use Rule
         None of the chemicals in this material have a SNUR under TSCA.
    SARA
       Section 302 (RQ)
         None of the chemicals in this material have an RQ.
       Section 302 (TPQ)
         None of the chemicals in this product have a TPQ.
       Section 313
         No chemicals are reportable under Section 313.
    Clean Air Act:
         This material does not contain any hazardous air pollutants.
         This material does not contain any Class 1 Ozone depletors.
         This material does not contain any Class 2 Ozone depletors.
    Clean Water Act:
         None of the chemicals in this product are listed as Hazardous
         Substances under the CWA.
         None of the chemicals in this product are listed as Priority
         Pollutants under the CWA.
         None of the chemicals in this product are listed as Toxic Pollutants
         under the CWA.
    OSHA:
         None of the chemicals in this product are considered highly hazardous
         by OSHA.
STATE
    C.I. Acid Blue 74 is not present on state lists from CA, PA, MN, MA,
    FL, or NJ.
    California No Significant Risk Level:
    None of the chemicals in this product are listed.
European/International Regulations
    European Labeling in Accordance with EC Directives
         Hazard Symbols: XN
         Risk Phrases:
                      R 22  Harmful if swallowed.
         Safety Phrases:
  WGK (Water Danger/Protection)
         CAS# 860-22-0: 1
  United Kingdom Occupational Exposure Limits

  Canada
         CAS# 860-22-0 is listed on Canada's DSL List.
         This product has a WHMIS classification of Not controlled..
         CAS# 860-22-0 is not listed on Canada's Ingredient Disclosure List.
  Exposure Limits

                 **** SECTION 16 - ADDITIONAL INFORMATION ****

    MSDS Creation Date: 12/02/1998  Revision #1 Date:  8/02/2000

    The information above is believed to be accurate and represents the best
    information currently available to us. However, we make no warranty of
    merchantability or any other warranty, express or implied, with respect to
    such information, and we assume no liability resulting from its use. Users
    should make their own investigations to determine the suitability of the
    information for their particular purposes. In no way shall the company be
    liable for any claims, losses, or damages of any third party or for lost
    profits or any special, indirect, incidental, consequential or exemplary
    damages, howsoever arising, even if the company has been advised of
    the possibility of such damages.