**** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION ****

MSDS Name: Aniline Blue, certified biological stain
Catalog Numbers:
    A967-25, S75043
Synonyms:
    Acid Blue B; Calcocid Blue B; Marine Blue V; China Blue; C.I. 42755;
    C.I. Acid Blue 22; Ink Blue A; Soluble Blue B; Benezenesulfonic
    acid,
Company Identification:  Fisher Scientific
                        1 Reagent Lane
                        Fairlawn, NJ  07410
For information, call:  201-796-7100
Emergency Number:       201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887

         **** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
|      CAS#      |              Chemical Name           |    %     |  EINECS#  |
|----------------|--------------------------------------|----------|-----------|
|    28631-66-5  |C.I. Acid Blue 22                     |   100    | 249-113-9 |
+----------------+--------------------------------------+----------+-----------+
         Hazard Symbols: None Listed.
         Risk Phrases: None Listed.

                 **** SECTION 3 - HAZARDS IDENTIFICATION ****

                              EMERGENCY OVERVIEW
Appearance:  dark purple.
Caution! May cause respiratory tract irritation. The toxicological
properties of this material have not been fully investigated. May
cause methemoglobinemia. May cause eye and skin irritation.
Target Organs: Blood.

Potential Health Effects
    Eye:
         May cause eye irritation.  This product contains an anionic dye.
         Similar dyes have not caused injury to the cornea or conjunctiva in
         documented exposure cases with human or rabbit eyes.
    Skin:
         May cause skin irritation.
    Ingestion:
         May cause gastrointestinal irritation with nausea, vomiting and
         diarrhea. Low hazard for usual industrial handling.
    Inhalation:
         May cause respiratory tract irritation. Low hazard for usual
         industrial handling.
    Chronic:
         Prolonged or repeated skin contact may cause irritation. May cause
         methemoglobinemia, which is characterized by chocolate-brown colored
         blood, headache, weakness, dizziness, breath shortness, cyanosis
         (bluish skin due to deficient oxygenation of blood), rapid heart
         rate, unconsciousness and possible death.

                   **** SECTION 4 - FIRST AID MEASURES ****

    Eyes:
         Flush eyes with plenty of water for at least 15 minutes,
         occasionally lifting the upper and lower eyelids. Get medical aid.
    Skin:
         Get medical aid if irritation develops or persists. Wash clothing
         before reuse. Flush skin with plenty of soap and water.
    Ingestion:
         If victim is conscious and alert, give 2-4 cupfuls of milk or water.
         Never give anything by mouth to an unconscious person. Get medical
         aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and
         drink 2-4 cupfuls of milk or water.
    Inhalation:
         Remove from exposure to fresh air immediately. If not breathing,
         give artificial respiration. If breathing is difficult, give oxygen.
         Get medical aid.
    Notes to Physician:
         Treat symptomatically and

                 **** SECTION 5 - FIRE FIGHTING MEASURES ****

    General Information:
         As in any fire, wear a self-contained breathing apparatus in
         pressure-demand, MSHA/NIOSH (approved or equivalent), and full
         protective gear. During a fire, irritating and highly toxic gases
         may be generated by thermal decomposition or combustion. This
         material in sufficient quantity and reduced particle size is capable
         of creating a dust explosion.
    Extinguishing Media:
         Use extinguishing media most appropriate for the surrounding fire.
    Autoignition Temperature:615 deg C ( 1,139.00 deg F)
    Flash Point:           70 deg C ( 158.00 deg F)
    Explosion Limits, lower:Not available.
    Explosion Limits, upper:Not available.
    NFPA Rating: (estimated) Health: 1; Flammability: 1; Reactivity: 0

               **** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

    General Information: Use proper personal protective equipment as indicated
                         in Section 8.
    Spills/Leaks:
         Vacuum or sweep up material and place into a suitable disposal
         container. Clean up spills immediately, observing precautions in the
         Protective Equipment section. Avoid generating dusty conditions.
         Provide ventilation.

                  **** SECTION 7 - HANDLING and STORAGE ****

    Handling:
         Wash thoroughly after handling. Remove contaminated clothing and
         wash before reuse. Use with adequate ventilation. Avoid contact with
         eyes, skin, and clothing. Keep container tightly closed. Avoid
         breathing dust.
    Storage:
         Store in a cool, dry, well-ventilated area away from incompatible
         substances. Keep containers tightly closed.

         **** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

    Engineering Controls:
         Facilities storing or utilizing this material should be equipped
         with an eyewash facility and a safety shower. Use adequate
         ventilation to keep airborne concentrations low.

                                Exposure Limits
+--------------------+-------------------+-------------------+-----------------+
|   Chemical Name    |        ACGIH      |       NIOSH       |OSHA - Final PELs|
|--------------------|-------------------|-------------------|-----------------|
| C.I. Acid Blue 22  |none listed        |none listed        |none listed      |
+--------------------+-------------------+-------------------+-----------------+

    OSHA Vacated PELs:
         C.I. Acid Blue 22:
            No OSHA Vacated PELs are listed for this chemical.

    Personal Protective Equipment

                Eyes:
                      Wear appropriate protective eyeglasses or chemical
                      safety goggles as described by OSHA's eye and face
                      protection regulations in 29 CFR 1910.133 or European
                      Standard EN166.
                Skin:
                      Wear appropriate protective gloves to prevent skin
                      exposure.
            Clothing:
                      Wear appropriate protective clothing to prevent skin
                      exposure.
         Respirators:
                      A respiratory protection program that meets OSHA's 29
                      CFR :1910.134 and ANSI Z88.2 requirements or European
                      Standard EN 149 must be followed whenever workplace
                      conditions warrant a respirator's use.

            **** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State:             Solid
Appearance:                 dark purple
Odor:                       odorless
pH:                         Not available.
Vapor Pressure:             Negligible.
Vapor Density:              Not available.
Evaporation Rate:           Negligible.
Viscosity:                  Not available.
Boiling Point:              Not available.
Freezing/Melting Point:     Not available.
Decomposition Temperature:  Not available.
Solubility in water:        Soluble.
Specific Gravity/Density:   Not available.
Molecular Formula:          C32H25N3O9S3Na2
Molecular Weight:           737.76

                **** SECTION 10 - STABILITY AND REACTIVITY ****

    Chemical Stability:
         Stable under normal temperatures and pressures.
    Conditions to Avoid:
         Dust generation.
    Incompatibilities with Other Materials:
         Strong oxidizing agents.
    Hazardous Decomposition Products:
         Nitrogen oxides, carbon monoxide, oxides of sulfur, carbon dioxide.
    Hazardous Polymerization: Has not been reported.

               **** SECTION 11 - TOXICOLOGICAL INFORMATION ****

    RTECS#:
         CAS# 28631-66-5: DB4958000
    LD50/LC50:
         Not available.
    Carcinogenicity:
      C.I. Acid Blue 22 -
         Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
    Epidemiology:
         No information available.
    Teratogenicity:
         No information available.
    Reproductive Effects:
         No information available.
    Neurotoxicity:
         No information available.
    Mutagenicity:
         EPA Genetox Program 1988, inconclusive: B subtilis rec assay.
    Other Studies:
         No data available.

                 **** SECTION 12 - ECOLOGICAL INFORMATION ****
 
 

                **** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Chemical waste generators must determine whether a discarded chemical
is classified as a hazardous waste.
US EPA guidelines for the classification determination are listed in
40 CFR Parts 261.3. Additionally, waste generators must consult state
and local hazardous waste regulations to ensure complete and accurate
classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

                 **** SECTION 14 - TRANSPORT INFORMATION ****

    US DOT
         No information available
    Canadian TDG
         No information available.

                 **** SECTION 15 - REGULATORY INFORMATION ****

US FEDERAL
    TSCA
         CAS# 28631-66-5 is listed on the TSCA inventory.
       Health & Safety Reporting List
         None of the chemicals are on the Health & Safety Reporting List.
       Chemical Test Rules
         None of the chemicals in this product are under a Chemical Test Rule.
       Section 12b
         None of the chemicals are listed under TSCA Section 12b.
       TSCA Significant New Use Rule
         None of the chemicals in this material have a SNUR under TSCA.
    SARA
       Section 302 (RQ)
         None of the chemicals in this material have an RQ.
       Section 302 (TPQ)
         None of the chemicals in this product have a TPQ.
       SARA Codes
         CAS # 28631-66-5: acute.
       Section 313
         No chemicals are reportable under Section 313.
    Clean Air Act:
         This material does not contain any hazardous air pollutants.
         This material does not contain any Class 1 Ozone depletors.
         This material does not contain any Class 2 Ozone depletors.
    Clean Water Act:
         None of the chemicals in this product are listed as Hazardous
         Substances under the CWA.
         None of the chemicals in this product are listed as Priority
         Pollutants under the CWA.
         None of the chemicals in this product are listed as Toxic Pollutants
         under the CWA.
    OSHA:
         None of the chemicals in this product are considered highly hazardous
         by OSHA.
STATE
    C.I. Acid Blue 22 is not present on state lists from CA, PA, MN, MA,
    FL, or NJ.
    California No Significant Risk Level:
    None of the chemicals in this product are listed.
European/International Regulations
    European Labeling in Accordance with EC Directives
         Hazard Symbols: Not available.
         Risk Phrases:
         Safety Phrases:
  WGK (Water Danger/Protection)
         CAS# 28631-66-5: No information available.
  United Kingdom Occupational Exposure Limits

  Canada
         CAS# 28631-66-5 is listed on Canada's DSL List.
         This product has a WHMIS classification of Not controlled..
         CAS# 28631-66-5 is not listed on Canada's Ingredient Disclosure List.
  Exposure Limits

                 **** SECTION 16 - ADDITIONAL INFORMATION ****

    MSDS Creation Date: 12/12/1997  Revision #3 Date:  8/29/2001

    The information above is believed to be accurate and represents the best
    information currently available to us. However, we make no warranty of
    merchantability or any other warranty, express or implied, with respect to
    such information, and we assume no liability resulting from its use. Users
    should make their own investigations to determine the suitability of the
    information for their particular purposes. In no way shall the company be
    liable for any claims, losses, or damages of any third party or for lost
    profits or any special, indirect, incidental, consequential or exemplary
    damages, howsoever arising, even if the company has been advised of
    the possibility of such damages.