**** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION ****
MSDS Name: Hematoxylin Stain Solution (Harris-Modified)
Catalog Numbers:
S0-H-20
Synonyms:
Company Identification: Fisher Scientific
1 Reagent Lane
Fairlawn, NJ 07410
For information, call: 201-796-7100
Emergency Number: 201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887
**** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****
+----------------+--------------------------------------+----------+-----------+
| CAS# | Chemical Name | % | EINECS# |
|----------------|--------------------------------------|----------|-----------|
| 64-19-7 |Acetic acid | 3.0 | 200-580-7 |
|----------------|--------------------------------------|----------|-----------|
| 67-63-0 |Isopropyl alcohol | 7.3 | 200-661-7 |
|----------------|--------------------------------------|----------|-----------|
| 517-28-2 |Hematoxylin | <1.0 | 208-237-3 |
|----------------|--------------------------------------|----------|-----------|
| 7732-18-5 |Water | Balance | 231-791-2 |
|----------------|--------------------------------------|----------|-----------|
| 7784-31-8 |Aluminum sulfate | 8.0 | unlisted |
|----------------|--------------------------------------|----------|-----------|
| 21908-53-2 |Mercuric oxide | <1.0 | 244-654-7 |
+----------------+--------------------------------------+----------+-----------+
Hazard Symbols: F
Risk Phrases: 11
**** SECTION 3 - HAZARDS IDENTIFICATION ****
EMERGENCY OVERVIEW
Highly flammable.
Potential Health Effects
Eye:
May cause eye irritation.
Skin:
May cause skin irritation. May cause skin sensitization, an allergic
reaction, which becomes evident upon re-exposure to this material.
Prolonged and/or repeated contact may cause defatting of the skin
and dermatitis. May be harmful if absorbed through the skin.
Ingestion:
May cause irritation of the digestive tract. May cause systemic
toxicity with acidosis. May cause liver and kidney damage. May cause
central nervous system depression, characterized by excitement,
followed by headache, dizziness, drowsiness, and nausea. Advanced
stages may cause collapse, unconsciousness, coma and possible death
due to respiratory failure. May cause cardiac disturbances.
Inhalation:
Inhalation of high concentrations may cause central nervous system
effects characterized by nausea, headache, dizziness, unconsciousness
and coma. May cause respiratory tract irritation. May cause effects
similar to those described for ingestion. Heated or misted substance
may cause headache, irregular eye movements, and possible coma.
Chronic:
Prolonged or repeated skin contact may cause dermatitis. Chronic
inhalation and ingestion may cause effects similar to those of acute
inhalation and ingestion.
**** SECTION 4 - FIRST AID MEASURES ****
Eyes:
Flush eyes with plenty of water for at least 15 minutes,
occasionally lifting the upper and lower eyelids. Get medical aid
immediately.
Skin:
Flush skin with plenty of soap and water for at least 15 minutes
while removing contaminated clothing and shoes. Get medical aid if
irritation develops or persists.
Ingestion:
If victim is conscious and alert, give 2-4 cupfuls of milk or water.
Never give anything by mouth to an unconscious person. Get medical
aid immediately.
Inhalation:
Remove from exposure to fresh air immediately. If not breathing,
give artificial respiration. If breathing is difficult, give oxygen.
Get medical aid.
Notes to Physician:
Urine acetone test may be helpful in diagnosis.
**** SECTION 5 - FIRE FIGHTING MEASURES ****
General Information:
As in any fire, wear a self-contained breathing apparatus in
pressure-demand, MSHA/NIOSH (approved or equivalent), and full
protective gear. Vapors may form an explosive mixture with air. Use
water spray to keep fire-exposed containers cosp; Will be easily ignited by heat, sparks or flame.
Extinguishing Media:
For small fires, use dry chemical, carbon dioxide, water spray or
alcohol-resistant foam. Use water spray to cool fire-exposed
containers. Water may be ineffective. Do NOT use straight streams of
water. For large fires, use dry chemical, carbon dioxide,
alcohol-resistant foam, or water spray.
**** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****
General Information: Use proper personal protective equipment as indicated
in Section 8.
Spills/Leaks:
Absorb spill with inert material (e.g. vermiculite, sand or earth),
then place in suitable container. Clean up spills immediately,
observing precautions in the Protective Equipment section. Remove all
sources of ignition. Provide ventilation. A vapor suppressing foam
may be used to reduce vapors. Water spray may reduce vapor but may
not prevent ignition in closed spaces.
**** SECTION 7 - HANDLING and STORAGE ****
Handling:
Wash thoroughly after handling. Use only in a well-ventilated area.
Use with adequate ventilation. Use spark-proof tools and explosion
proof equipment. Avoid contact with eyes, skin, and clothing. Empty
containers retain product residue, (liquid and/or vapor), and can be
dangerous. Keep container tightly closed. Avoid contact with heat,
sparks and flame. Avoid ingestion and inhalation. Do not pressurize,
cut, weld, braze, solder, drill, grind, or expose empty containers
to heat, sparks or open flames.
Storage:
Keep away from heat, sparks, and flame. Keep away from sources of
ignition. Store in a cool, dry place. Store in a tightly closed
container. Store in a cool, dry, well-ventilated area away from
incompatible substances. Regularly check inhibitor levels to
maintain peroxide levels below 1%. After opening, purge container
with nitrogen before reclosing. Periodically test for peroxide
formation on long-term storage. Addition of water or appropriate
reducing materials will lessen peroxide formation.
**** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****
Engineering Controls:
Use adequate general or local exhaust ventilation to keep airborne
concentrations below the permissible exposure limits.
Personal Protective Equipment
Eyes:
Wear appropriate protective eyeglasses or chemical
safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European
Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin
exposure.
Clothing:
Wear appropriate protective clothing to prevent skin
exposure.
Respirators:
Follow the OSHA respirator regulations found in 29CFR
1910.134 or European Standard EN 149. Always use a
NIOSH or European Standard EN 149 approved respirator
when necessary.
**** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****
Physical State: Liquid
Appearance: Not available.
Odor: Not available.
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate: Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point: Not available.
Autoignition Temperature: Not available.
Flash Point: 130 deg F ( 54.44 deg C)
Explosion Limits, lower: Not available.
Explosion Limits, upper: Not available.
Decomposition Temperature:
Solubility:
Specific Gravity/Density:
Molecular Formula:
Molecular Weight:
**** SECTION 10 - STABILITY AND REACTIVITY ****
Chemical Stability:
Stable under normal temperatures and pressures. This material may be
sensitive to peroxide formation.
Conditions to Avoid:
High temperatures, mechanical shock, incompatible materials, ignition
sources, excess heat.
Incompatibilities with Other Materials:
&ne, propylamine, pyridine,
triethylamine), azo, diazo, and hydrazines (e.g. dimethyl hydrazine,
hydrazine, methyl hydrazine), caustics (e.g. ammonia, ammonium
hydroxide, calcium hydroxide, potassium hydroxide, sodium
hydroxide), cyanides (e.g. potassium cyanide, sodium cyanide),
dithiocarbamates (e.g. ferbam, maneb, metham, thiram), fluorides
(inorganic, e.g. ammonium fluoride, calcium fluoride, cesium
fluoride), isocyanates (e.g. methyl isocyanate), metals (alkali and
alkaline, e.g. cesium, potassium, sodium), metals as powders (e.g.
hafnium, raney nickel), metals and metal compounds (toxic, e.g.
beryllium, lead acetate, nickel carbonyl, tetraethyl lead), nitrides
(e.g. potassium nitride, sodium nitride), nitriles (e.g.
acetonitrile, methyl cyanide), sulfides (inorganic, e.g. ferric
sulfide, lead sulfide, sodium sulfide), epoxides (e.g. butyl
glycidyl ether), explosives (e.g. ammonium nitrate, hydrazoic acid,
sodium azide), polymerizable compounds (e.g. butadiene, methyl
acrylate, styrene, vinyl chloride), oxidizing agents (strong, e.g.
bromine, hydrogen peroxide, nitrogen dioxide, potassium nitrate),
reducing agents (strong, e.g. aluminum carbide, chlorosilane,
hydrogen phosphide, lithium hydride), water reactive substances (e.g.
acetic anyhdride, alkyl aluminum chloride, calcium carbide, ethyl
dichlorosilane), acids (mineral, non-oxidizing, e.g. hydrochloric
acid, hydrofluoric acid, muriatic acid, phosphoric acid), acids
(organic, e.g. acetic acid, benzoic acid, formic acid, methanoic
acid, oxalic acid), peroxides and hydroperoxides (organic, e.g.
acetyl peroxide, benzoyl peroxide, butyl peroxide, methyl ethyl
ketone peroxide), This material has been reported to be susceptib.
Hazardous Decomposition Products:
Carbon monoxide, oxides of sulfur, irritating and toxic fumes and
gases, carbon dioxide, oxides of carbon.
Hazardous Polymerization: Has not been reported
**** SECTION 11 - TOXICOLOGICAL INFORMATION ****
RTECS#:
CAS# 64-19-7: AF1225000
CAS# 67-63-0: NT8050000
CAS# 517-28-2: MH7875000
CAS# 7732-18-5: ZC0110000
CAS# 7784-31-8: WS5697000
CAS# 21908-53-2: OW8750000
LD50/LC50:
CAS# 64-19-7: Draize test, rabbit, skin: 50 mg/24H Mild; Inhalation,
mouse: LC50 = 5620 ppm/1H; Oral, rat: LD50 = 3310 mg/kg; Skin,
rabbit: LD50 = 1060 uL/kg.
CAS# 67-63-0: Draize test, rabbit, eye: 100 mg Severe; Draize test,
rabbit, eye: 10 mg Moderate; Draize test, rabbit, eye: 100 mg/24H
Moderate; Draize test, rabbit, skin: 500 mg Mild; Inhalation, rat:
LC50 = 16000 ppm/8H; Oral, mouse: LD50 = 3600 mg/kg; Oral, rabbit:
LD50 = 6410 mg/kg; Oral, rat: LD50 = 5045 mg/kg; Skin, rabbit: LD50
= 12800 mg/kg.
CAS# 517-28-2.
CAS# 7732-18-5: Oral, rat: LD50 = >90 mL/kg.
CAS# 7784-31-8: Oral, mouse: LD50 = >9 gm/kg; Oral, rat: LD50 = >9
gm/kg.
CAS# 21908-53-2: Oral, mouse: LD50 = 16 mg/kg; Oral, rat: LD50 = 18
mg/kg; Skin, rat: LD50 = 315 mg/kg.
Carcinogenicity:
Acetic acid -
Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Isopropyl alcohol -
IARC: Group 3 carcinogen
Hematoxylin -
Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Water -
Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Aluminum sulfate -
Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
Mercuric oxide -
ACGIH: A4 - not classifiable as a human carcinogen (listed as **
IARC: Group 3 carcinogen (listed as ** undefined **).
Epidemiology:
Early epidemiological studies suggested an association between the
strong acid manufacture of isopropyl alcohol and paranasal sinus
cancer in workers. The risk of laryngeal cancer may also be increased
in these workers. However, it has not been tested adequately in
animals to assess its carcinogenicity.
Teratogenicity:
No data available.
Reproductive Effects:
orl-rat TDLo:11340 mg/kg (45D pre) orl-rat TDLo:5040 mg/kg (1-20D
preg) orl-rat TDLo:20160 mg/kg (1-20D preg) orl-rat TDLo:32400 5g/kg
(26W pre) ihl-rat TCLo:3500 ppm/7H (1-19D preg) ihl-rat TCLo:10000
ppm/7H (1-19D preg) ihl-rat TCLo:7000 ppm/7H (1-19D preg) orl-man
TDLo:14432 mg/kg orl-hmn TDLo:223 mg/kg orl-man LDLo:5272 mg/kg
orl-hmn LDLo:3570 mg/kg
Neurotoxicity:
No data available.
Mutagenicity:
No data available.
Other Studies:
&nbs 13 - DISPOSAL CONSIDERATIONS ****
Dispose of in a manner consistent with federal, state, and local regulations.
**** SECTION 14 - TRANSPORT INFORMATION ****
US DOT
No information available
Canadian TDG
No information available.
**** SECTION 15 - REGULATORY INFORMATION ****
US FEDERAL
TSCA
CAS# 64-19-7 is listed on the TSCA inventory.
CAS# 67-63-0 is listed on the TSCA inventory.
CAS# 517-28-2 is listed on the TSCA inventory.
CAS# 7732-18-5 is listed on the TSCA inventory.
CAS# 7784-31-8 is not listed on the TSCA inventory.
It is for research and development use only.
CAS# 21908-53-2 is listed on the TSCA inventory.
This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 64-19-7 is listed as a Hazardous Substance under the CWA.
CAS# 21908-53-2 is listed as a Priority Pollutant under the Clean
Water Act.
CAS# 21908-53-2 is listed as a Toxic Pollutant under the Clean Water
Act.
OSHA:
None of the chemicals in this product are considered highly hazardous
by OSHA.
STATE
Acetic acid can be found on the following state right to know lists:
California, New Jersey, Florida, Pennsylvania, Minnesota,
Massachusetts.
Isopropyl alcohol can be found on the following state right to know
lists: California, New Jersey, Florida, Pennsylvania, Minnesota,
Massachusetts.
Hematoxylin is not present on state lists from CA, PA, MN, MA, FL, or
NJ.
Water is not present on state lists from CA, PA, MN, MA, FL, or NJ.
Aluminum sulfate can be found on the following state right to know
lists: California, (listed as ** no name **), New Jersey, (listed as
** no name **), Florida, (listed as ** no name **), Pennsylvania,
(listed as ** no name **), Minnesota, (listed as ** no name **),
Massachusetts, (listed as ** no name **).
Mercuric oxide can be found on the following state right to know
lists: California, (listed as ** no name **), California, (listed as
** no name **), New Jersey, Florida, Pennsylvania, Minnesota, (listed
as ** no name **), Massachusetts.
WARNING: This product contains Mercuric oxide, listed as ` **
undefined **', a chemical known to the state of California to cause
birth defects or other reproductive harm.
California No Significant Risk Level:
None of the chemicals in this product are listed.
European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: F
Risk Phrases:
R 11 Highly flammable.
Safety Phrases:
S 7 Keep container tightly closed.
S 16 Keep away from sources of ignition - No
smoking.
WGK (Water Danger/Protection)
CAS# 64-19-7: 1
CAS# 67-63-0: 1
CAS# 517-28-2: 1
CAS# 7732-18-5: No information available.
CAS# 7784-31-8: No information available.
CAS# 21908-53-2: 3
United Kingdom Occupational Exposure Limits
CAS# 64-19-7: OES-United Kingdom, TWA 10 ppm TWA; 25 mg/m3 TWA
CAS# 64-19-7: OES-United Kingdom, STEL 15 ppm STEL; 37 mg/m3 STEL
CAS# 67-63-0: OES-United Kingdom, TWA 400 ppm TWA; 999 mg/m3 TWA
CAS# 67-63-0: OES-United Kingdom, STEL 500 ppm STEL; 1250 mg/m3 STEL
CAS# 67-63-0: OES-United Kingdom, STEL 500 ppm STEL; 1250 mg/m3 STEL
CAS# 7784-31-8: OES-United Kingdom, TWA (listed as ** undefined
**): total inhalable dust: 10 mg/m3 TWA; respirable dust: 4 mg/m3
TWA
Canada
CAS# 64-19-7 is listed on Canada's DSL List.
CAS# 67-63-0 is listed on Canada's DSL List.
CAS# 517-28-2 is listed on Canada's DSL List.
CAS# 7732-18-5 is listed on Canada's DSL List.
CAS# 21908-53-2 is listed on Canada's DSL List.
CAS# 64-19-7 is listed on Canada's Ingredient Disclosure List.
CAS# 67-63-0 is listed on Canada's Ingredient Disclosure List.
CAS# 517-28-2 is not listed on Canada's Ingredient Disclosure List.
CAS# 7732-18-5 is not listed on Canada's Ingredient Disclosure List.
CAS# 7784-31-8 is not listed on Canada's Ingredient Disclosure List.
CAS# 21908-53-2 is listed on Canada's Ingredient Disclosure List.
Exposure Limits
CAS# 64-19-7: OEL-AUSTRALIA:TWA 10 ppm (25 mg/m3);STEL 15 ppm (37 mg/m
3)
OEL-AUSTRIA:TWA 10 ppm (25 mg/m3)
OEL-BELGIUM:TWA 10 ppm (25 mg/m3);STEL 15 ppm (37 mg/m3)
OEL-CZECHOSLOVAKIA:TWA 25 mg/m3;STEL 50 mg/m3
OEL-DENMARK:TWA 10 ppm (25 mg/m3)
OEL-FINLAND:TWA 10 ppm (25 mg/m3);STEL 15 ppm (37 mg/m3);Skin
OEL-THE PHILIPPINES:TWA 10 ppm (25 mg/m3)
OEL-POLAND:TWA 5 mg/m3
OEL-RUSSIA:TWA 10 ppm;STEL 5 mg/m3;Skin
OEL-SWEDEN:TWA 10 ppm (25 mg/m3);STEL 15 ppm (35 mg/m3)
OEL-SWITZERLAND:TWA 10 ppm (25 mg/m3);STEL 20 ppm (50 mg/m3)
OEL-THAILAND:TWA 10 ppm (25 mg/m3)
OEL-TURKEY:TWA 10 ppm (25 mg/m3)
OEL-UNITED KINGDOM:TWA 10 ppm (25 mg/m3);STEL 15 ppm (35 mg/m3)
OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV
OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGI TLV
CAS# 67-63-0: OEL-AUSTRALIA:TWA 400 ppm (980 mg/m3);STEL 500 ppm (1225
mg/m3)
OEL-BELGIUM:TWA 400 ppm (985 mg/m3);STEL 500 ppm (1230 mg/m3)
OEL-DENMARK:TWA 200 ppm (490 mg/m3);Skin
OEL-FRANCE:STEL 400 ppm (980 mg/m3)
OEL-GERMANY:TWA 400 ppm (980 mg/m3)
OEL-JAPAN:STEL 400 ppm (980 mg/m3)
OEL-THE NETHERLANDS:TWA 400 ppm (980 mg/m3);Skin
OEL-THE PHILIPPINES:TWA 400 ppm (980 mg/m3)
OEL-RUSSIA:STEL 400 ppm (10 mg/m3)
OEL-SWEDEN:TWA 150 ppm (350 mg/m3);STEL 250 ppm (600 mg/m3)
OEL-SWITZERLAND:TWA 400 ppm (980 mg/m3);STEL 800 ppm
OEL-TURKEY:TWA 200 ppm (500 mg/m3)
OEL-UNITED KINGDOM:TWA 400 ppm (980 mg/m3);STEL 500 ppm;Skin
OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV
OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGI TLV
CAS# 7784-31-8 (listed as ** undefined **): OEL-AUSTRALIA:TWA 10 mg/m3
CAS# 21908-53-2: OEL-AUSTRALIA:TWA 0.05 mg(Hg)/m3;Skin JANUARY 1993
OEL-BELGIUM:TWA 0.05 mg(Hg)/m3;Skin JANUARY 1993
OEL-CZECHOSLOVAKIA:TWA 0.05 mg(Hg)/m3;STEL 0.15 mg(Hg)/m3
OEL-DENMARK:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-FINLAND:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-FRANCE:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-GERMANY:TWA 0.01 ppm (0.1 mg(Hg)/m3) JANUARY 1993
OEL-HUNGARY:TWA 0.02 mg(Hg)/m3;STEL 0.04 mg(Hg)/m JANUARY 1993
OEL-JAPAN:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-THE NETHERLANDS:TWA 0.05 mg(Hg)/m3;STEL 0.15 mg(Hg)/m3
OEL-THE PHILIPPINES:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-POLAND:TWA 0.01 mg(Hg)/m3 JANUARY 1993
OEL-RUSSIA:TWA 0.05 mg(Hg)/m3;STEL 0.01 mg(Hg)/m3 JANUARY 1993
OEL-SWEDEN:TWA 0.05 mg(Hg)/m3 JANUARY 1993
OEL-THAILAND:STEL 0.05 mg(Hg)/m3 JANUARY 1993
OEL-UNITED KINGDOM:TWA 0.05 mg(Hg)/m3;STEL 0.15 mg(Hg)/m3
OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV
OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGI TLV
**** SECTION 16 - ADDITIONAL INFORMATION ****
MSDS Creation Date: 9/22/1998 Revision #2 Date: 8/02/2000
The information above is believed to be accurate and represents the best
information currently available to us. However, we make no warranty of
merchantability or any other warranty, express or implied, with respect to
such information, and we assume no liability resulting from its use. Users
should make their own investigations to determine the suitability of the
information for their particular purposes. In no way shall the company be
liable for any claims, losses, or damages of any third party or for lost
profits or any special, indirect, incidental, consequential or exemplary
damages, howsoever arising, even if the company has been advised of
the possibility of such damages.
--------------------------------------------------------------------------------